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KMID : 0043319900130040330
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Archives of Pharmacal Research
1990 Volume.13 No. 4 p.330 ~ p.337
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Study on Antitumor and Immunomodulating Activities of Polysaccharide Fractions from Panax ginseng: Comparison of effects of neutral and acidic polysaccharide fraction
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Kim YS
Kang KS/Kim SI
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Abstract
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The crude polysaccharide from Panax ginseng prepared by hot water extraction and precipitation with ethanol was further fractionated into neutral and acidic fractions by DEAE-cellulose ion exchange chromatography. The chemical compositions were 85.0% carbohydrate and 15.0% protein for the neutral fraction, and 28.4% carbohydrate, 10.0% protein and 29.0% uronic acid for the acidic fraction. The acidic fraction was more effective in increasing of the ratio of spleen to body weight, the number of antibody secreting cells to SRBC and phagocytic activity of reticuloendothelial system, as well as antitumor activity against the solid form of sarcoma 180 in ICR mice than the neutral fraction. All polysaccharide fractions were mitogenic to cultured spleen cells of C57BL/6 mice. However, FA was different from FN in the co-mitogenicities with lectin mitogens. Both crude and acidic fractions potentiated remarkably the mitogenic activity of PHA-P or LPS in dose-dependent manner but neutral fraction enhanced only that of LPS. Three polysaccharide fractions had no effect on that of Con A. These results suggest that the acidic fraction may stimulate B and T cells as well as macrophages while the neutral fraction may stimulate only B cells and macropages.
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